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For user control AutoScaleMode applies to the contents of the container that way controls within a form can be repositioned and scaled according the Form level AutoScaleMode and the user control contents are scaled according to the user control level AutoScaleMode. Below is the same form with user control with three different combinations of user control AutoScaleMode and FixedWidth/FixedHeight.

Comment	Starting form (Font 8)	Starting Form (Font 12)
Default UserControl location scaled size scaled UserControl Contents location scaled size scaled
UserControl.AutoSizeMode = None; UserControl location scaled size scaled UserControl Contents Location NOT scaled size NOT scaled
UserControl.AutoSizeMode = None; UserControl.ControlStyles FixedWidth/Height true UserControl location scaled size NOT scaled UserControl Contents location NOT scaled size NOT scaled